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排序方式: 共有238条查询结果,搜索用时 31 毫秒
101.
Artemis's Wil 《家用电脑与游戏机》2011,(2)
最近最火的游戏是什么?毫无疑问,是创造了全球最高在线人数记录的《魔兽世界:大地的裂变》,作为顶级的游戏外设设备制造厂商,赛睿也是看到了这个商机推出了大量的新品,其中Kinzu Pro红色版鼠标和MMO魔兽世界鼠标是赛睿的主推之作。 相似文献
102.
103.
Effects on Salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan 总被引:1,自引:0,他引:1
Leleu S Herman L Heyndrickx M De Reu K Michiels CW De Baerdemaeker J Messens W 《International journal of food microbiology》2011,145(1):43-48
Chitosan is a biopolymer with antimicrobial activity and film-forming properties. In this study, the effects on Salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan was evaluated. A chitosan was selected from eight types (four non-commercial and four commercial) based on its antimicrobial activity against Salmonella enterica serovar Enteritidis (S. Enteritidis). For this purpose, a contact plate method was developed and chitosans were applied at a concentration of 0.25% (w/v). A commercial type with a molecular weight of 310-375 kDa and a deacetylation degree of 75% that reduced S. Enteritidis by 0.71 log10 colony forming units compared to the control (without chitosan) was selected for further studies. The chitosan was shown to have antimicrobial activity against other egg borne bacteria, i.e., Acinetobacter baumannii, Alcaligenes sp., Carnobacterium sp., Pseudomonas sp., Serratia marcescens and Staphylococcus warneri, and against S. enterica serovar Typhimurium, Escherichia coli and Listeria monocytogenes. The effects of various concentrations of the selected chitosan (0.25%, 1% and 2%) on Salmonella shell contamination and trans-shell penetration were assessed using the agar molding technique. Effective reduction of eggshell contamination could not be demonstrated, but trans-shell penetration was significantly reduced in the presence of a 2% chitosan eggshell coating, with only 6.1% of the eggs being penetrated compared to 24.5% of the uncoated eggs. It was concluded that the 2% chitosan coating has the potential to reduce contamination of egg contents resulting from trans-shell penetration by S. Enteritidis. 相似文献
104.
Bacterial inactivation by high-pressure homogenisation and high hydrostatic pressure 总被引:2,自引:0,他引:2
The resistance of five gram-positive bacteria, Enterococcus faecalis, Staphylococcus aureus, Lactobacillus plantarum, Listeria innocua and Leuconostoc dextranicum, and six gram-negative bacteria, Salmonella enterica serovar typhimurium, Shigella flexneri, Yersinia enterocolitica, Pseudomonas fluorescens and two strains of Escherichia coli, to high-pressure homogenisation (100-300 MPa) and to high hydrostatic pressure (200-400 MPa) was compared in this study. Within the group of gram-positive bacteria and within the group of gram-negative bacteria, large differences were observed in resistance to high hydrostatic pressure, but not to high-pressure homogenisation. All gram-positive bacteria were more resistant than any of the gram-negative bacteria to high-pressure homogenisation, while in relative to high hydrostatic pressure resistance both groups overlapped. Within the group of gram-negative bacteria, there also existed another order in resistance to high-pressure homogenisation than to high hydrostatic pressure. Further it appears that the mutant E. coli LMM1010, which is resistant to high hydrostatic pressure is not more resistant to high-pressure homogenisation than its parental strain MG1655. The preceding observations indicate a different response of the test bacteria to high-pressure homogenisation compared to high hydrostatic pressure treatment, which suggests that the underlying inactivation mechanisms for both techniques are different. Further, no sublethal injury could be observed upon high-pressure homogenisation of Y. enterocolitica and S. aureus cell population by using low pH (5.5 7), NaCl (0 6%) or SDS (0-100 mg/l) as selective components in the plating medium. Finally, it was observed that successive rounds of high-pressure homogenisation have an additive effect on viability reduction of Y. enterocolitica and S. aureus. 相似文献
105.
Dutch national planning has acquired an international reputation because it provides strong planning guidance while simultaneously being responsive to the particular spatial and political circumstances of different regions and areas. Spatial concepts, like the Randstad, are important vehicles for sustaining this approach. Such concepts incorporate select spatial planning rationales that justify operational decisions. Concepts can, however, also be ambiguous, and this can allow for different interpretations and deliberations about how guidance should take effect in different situations. In this paper we assess the degree of ambiguity contained in concepts outlined in Dutch national plans between 1988 and 2012. By focusing on the dimensions of spatial concepts, and the room for interpretation these create, we demonstrate how concepts were modified to accommodate a shifting appreciation of deliberation and, as a result, collaboration and governance. On a theoretical level, we propose a method that analyses in detail the ambiguity (“fuzzyness” or “softness”) of spatial concepts. We argue that such sophisticated understandings contribute to explaining the variety of governance responses that these geographies produce in practice. On an empirical level we seek to increase understanding of change in recent Dutch national planning. 相似文献
106.
107.
A novel method for the determination of controller parameters in a broad class of linear control systems affected by time-delays is presented. This method is based on an appropriate shaping of the spectrum of the closed-loop system. Its application follows two steps. First, a number of rightmost poles, smaller than the number of controller parameters, are directly assigned. This leads to constraints on the controller parameters. By using algebraic techniques a complete parametrization of all controllers satisfying these constraints is obtained. In the second step, the remaining degrees of freedom in the parameter space are used to shift the remaining part of the system spectrum as far to the left as possible. This corresponds to an optimization problem involving a nonsmooth, non-convex objective function. An extensive real-plant example is presented to demonstrate the effectiveness and applicability of the procedure. 相似文献
108.
Riccardo Marega Federica De Leo Florent Pineux Jacopo Sgrignani Alessandra Magistrato Anil Damodar Naik Yann Garcia Lionel Flamant Carine Michiels Davide Bonifazi 《Advanced functional materials》2013,23(25):3173-3184
With the aim to design addressable magnetically‐active carbon nanotubes (CNTs) for cancer treatment, the use of Fe‐filled CNTs (Fe@MWCNTs) as multifunctional scaffolds is reported for exohedrally anchoring a monoclonal antibody (mAb) known to bind a plasma membrane receptor over‐expressed in several cancer cells (EGFR). Comprehensive microscopic (transmission electron microscopy, atomic force microscopy, and scanning electron microscopy) and spectroscopic (Raman, 57Fe Mossbauer, energy dispersive spectroscopy, X‐ray photoelectron spectroscopy (XPS), X‐ray diffraction) characterizations reveal the efficient confinement of magnetically‐active Fe phases (α‐Fe and Fe3C), while compositional evaluations through XPS, thermogravimetric analysis and gel electrophoresis confirm that mAb immobilization onto Fe@MWCNTs occurs. Enzyme‐linked immunosorbent assay (ELISA), confocal microscopy imaging and western blotting confirm the targeting action toward EGFR‐overexpressing cell lines (EGFR+). In vitro magnetic filtration experiments demonstrate that a selective removal of EGFR+ cells from a mixed population of healthy cell lines could be obtained in very short times (≈10 min). Cytotoxicity evaluations by classic cell staining procedures after application of an electromagnetic radiation inducing magnetic fluid hyperthermia (MFH), show a selective suppression of the EGFR+ cell line. Molecular dynamics and docking simulations of the hybrid mAb/Fe@MWCNTs conjugates nicely show how the presence of the CNT framework does not sterically affect the conformational properties of the two antigen binding regions, further supporting the biochemical findings. 相似文献
109.
Wil V. Srubar III Srikanth Pilla Zachary C. Wright Cecily A. Ryan Joseph P. Greene Curtis W. Frank Sarah L. Billington 《Composites Science and Technology》2012
Fully biobased composite materials were fabricated using a natural, lignocellulosic filler, namely oak wood flour (OWF), as particle reinforcement in a biosynthesized microbial polyester matrix derived from poly(β-hydroxybutyrate)-co-poly(β-hydroxyvalerate) (PHBV) via an extrusion injection molding process. The mechanisms and effects of processing, filler volume percent (vol%), a silane coupling agent, and a maleic anhydride (MA) grafting technique on polymer and composite morphologies and tensile mechanical properties were investigated and substantiated through calorimetry testing, scanning electron microscopy, and micromechanical modeling of initial composite stiffness. The addition of 46 vol% silane-treated OWF improved the tensile modulus of neat PHBV by 165%. Similarly, the tensile modulus of MA-grafted PHBV increased 170% over that of neat PHBV with a 28 vol% addition of untreated OWF. Incorporation of OWF reduced the overall degree of crystallinity of the matrix phase and induced embrittlement in the composites, which led to reductions in ultimate tensile stress and strain for both treated and untreated specimens. Deviations from the Halpin–Tsai/Tsai–Pagano micromechanical model for composite stiffness in the silane and MA compatibilized specimens are attributed to the inability of the model both to incorporate improved dispersion and wettability due to fiber–matrix modifications and to account for changes in neat PHBV and MA-grafted PHBV polymer morphology induced by the OWF. 相似文献
110.
C. Nouvellon M. Michiels J.P. Dauchot C. Archambeau F. Laffineur E. Silberberg S. Delvaux R. Cloots S. Konstantinidis R. Snyders 《Surface & coatings technology》2012
In this study, reactive High Power Impulse Magnetron Sputtering (HiPIMS) experiments were carried out to synthesize titanium oxide films, using a 45 × 15 cm² titanium target in Ar/O2 gas mixtures. The deposition process was studied as a function of the peak current (ipeak) at constant voltage during the pulse (1 kV) and constant average power (Pav). As the oxygen flow was increased, ipeak was kept constant (160, 300 or 400A) by adjusting the pulse duration and the average power (2 or 4 kW) by adjusting the pulse repetition frequency. For all experimental conditions, an abrupt transition from metallic towards poisoned regimes was observed. The transition curves exhibit hysteresis. As ipeak is increased from 160 A to 450 A, for Pav = 4 kW, the oxygen content (Ω) in the Ar/O2 mixture needed to poison the target surface was reduced from Ω = 11.5% to Ω = 8.5%. These values are much smaller than those recorded for DC magnetron sputtering (DCMS) (Ω = 42%) and pulsed DCMS (Ω = 36%) experiments carried out at the same power. These results are explained by the enhancement of the ionization and dissociation rates of oxygen molecules with the increase of ipeak. 相似文献